Fujii M, Goto N
Department of Otolaryngology, Nihon University School of Medicine, Tokyo, Japan.
Ann Otol Rhinol Laryngol. 1989 Sep;98(9):732-6. doi: 10.1177/000348948909800914.
Nerve fiber analyses were conducted on the human facial nerve with use of a new staining method (Luxol-PAS-hematoxylin stain; discriminative staining method) that permits simultaneous observation of the axon and surrounding myelin sheath. The following combination of equipment was employed in the study: an image-analyzing digitizer, a microscope with a drawing tube, and a computer for storing the data and performing statistical analyses. The numbers, transverse areas, and circularity ratios of axons were measured in 11 cases. The average number of axons composing one facial nerve was 6,254, and the average size of the axons was 6.23 microns 2. The results indicated that although the numbers of axons became reduced with age, the transverse areas and circularity ratios of the axons did not change with age.
采用一种新的染色方法（卢氏酚-过碘酸-苏木精染色法；鉴别染色法）对人类面神经进行神经纤维分析，该方法可同时观察轴突及其周围的髓鞘。本研究使用了以下设备组合：图像分析数字化仪、带绘图管的显微镜以及用于存储数据和进行统计分析的计算机。对11例病例的轴突数量、横截面积和圆度比进行了测量。构成一条面神经的轴突平均数量为6254个，轴突的平均大小为6.23平方微米。结果表明，虽然轴突数量随年龄增长而减少，但轴突的横截面积和圆度比并不随年龄变化。
