Tangen A, Lund W
University of Oslo, Department of Chemistry, Norway.
J Chromatogr A. 2000 Sep 1;891(1):129-38. doi: 10.1016/s0021-9673(00)00617-8.
A modified microconcentric nebulizer (MCN-100) has been used to construct an improved interface with minimised liquid and gas phase dead volumes for the coupling of capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICP-MS). A plate number of 3.6 x 10(6) plates m(-1) has been achieved. This is an order of magnitude better than the results previously reported for CE-ICP-MS. The separation efficiency of the system is demonstrated by the baseline separation of eight rare earth metals within a time span of 14.6 s. The system was used to control the purity of vitamin B12 and glutaredoxin 2 from Escherichia coli.
一种改进的微型同心雾化器（MCN - 100）已被用于构建一个改进的接口，该接口具有最小化的液相和气相死体积，用于毛细管电泳（CE）和电感耦合等离子体质谱（ICP - MS）的联用。已实现3.6×10⁶ 塔板数米⁻¹ 的塔板数。这比先前报道的CE - ICP - MS结果好一个数量级。该系统的分离效率通过在14.6秒的时间跨度内对八种稀土金属的基线分离得以证明。该系统用于控制来自大肠杆菌的维生素B12和谷氧还蛋白2的纯度。
