University of Auckland, School of Chemical Sciences, Private Bag 92019, Auckland 1142, New Zealand(1).
University of Auckland, School of Chemical Sciences, Private Bag 92019, Auckland 1142, New Zealand(1).
J Chromatogr A. 2018 Aug 3;1561:76-82. doi: 10.1016/j.chroma.2018.05.036. Epub 2018 May 16.
Capillary electrophoretic analyses benefit significantly from hyphenation to mass spectrometric techniques. While the coupling to ESI-MS is routinely performed, for example by using a coaxial sheath-flow interface, hyphenating it to inductively coupled plasma mass spectrometry is more technically challenging. We use a commercially available coaxial sheath-flow interface (CSFI) and a simple PTFE-based end-cap for easy, inexpensive CE-ICP-MS hyphenation with improved sensitivity and analytical performance compared to commercially available interfaces. We have optimized key nebulizer parameters such as capillary position, sheath liquid flow rate, and carrier gas flow rate, and compared the CSFI with a commercially available interface. In a set of proof-of-principle experiments employing the anticancer agent cisplatin it was demonstrated that the signal to noise response and sensitivity were considerably improved leading to detection limits for Pt of 0.08 μM.
毛细管电泳分析受益于与质谱技术的连接。虽然已经常规地进行了与 ESI-MS 的连接，例如通过使用同轴鞘流接口，但将其与电感耦合等离子体质谱连接更具技术挑战性。我们使用了一种市售的同轴鞘流接口（CSFI）和一个简单的基于 PTFE 的端盖，与市售接口相比，实现了 CE-ICP-MS 的简单、经济的连接，提高了灵敏度和分析性能。我们已经优化了关键的雾化器参数，如毛细管位置、鞘液流速和载气流速，并对 CSFI 与市售接口进行了比较。在一组使用抗癌药物顺铂的原理验证实验中，证明了信号噪声响应和灵敏度得到了显著提高，导致 Pt 的检测限为 0.08 μM。
