Deng B, Chan W T
Department of Chemistry, University of Hong Kong, China.
J Chromatogr A. 2000 Sep 1;891(1):139-48. doi: 10.1016/s0021-9673(00)00622-1.
A simple interface has been developed to couple capillary electrophoresis (CE) to inductively coupled plasma atomic emission spectrometry (ICP-AES) for metal speciation. A concentric glass nebulizer with elongated tip is used as the CE-ICP interface. The CE capillary is the central tube of the nebulizer. A platinum wire is wrapped across the exit end of the CE capillary to provide electrical connection to the CE power supply. No sheath flow of buffer solution is needed. A simple cooling system has also been developed. A peristaltic pump circulates water through a plastic tube that encloses the section of the CE capillary between the CE instrument and the ICP spectrometer. Characteristics of the CE-ICP interface, e.g., elution time, nebulization and transport efficiency and peak broadening, versus carrier gas flow-rate have been studied. Comparisons to a previous design with the Pt electrode inserted into the end of the CE capillary are made where appropriate. The reproducibility (RSD) in ICP emission intensity of the system is <4%. Detection limits of Cr and Cu are approximately 5 ng/ml.
已开发出一种简单的接口，用于将毛细管电泳（CE）与电感耦合等离子体原子发射光谱法（ICP-AES）联用进行金属形态分析。一种带有细长尖端的同心玻璃雾化器用作CE-ICP接口。CE毛细管是雾化器的中心管。一根铂丝缠绕在CE毛细管的出口端，以提供与CE电源的电连接。不需要缓冲溶液的鞘流。还开发了一种简单的冷却系统。蠕动泵使水通过一根塑料管循环，该塑料管围绕着CE毛细管在CE仪器和ICP光谱仪之间的部分。研究了CE-ICP接口的特性，如洗脱时间、雾化和传输效率以及峰展宽与载气流速的关系。在适当的地方与之前将铂电极插入CE毛细管末端的设计进行了比较。该系统ICP发射强度的重现性（相对标准偏差）<4%。Cr和Cu的检测限约为5 ng/ml。
