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[细胞悬液在正常条件下及暴露于外部因素时的光散射]

[Light scattering by cell suspensions in normal conditions and exposed to external factors].

作者信息

Ostashevskiĭ I Ia, Sungurov A Iu, Volchkov V A

出版信息

Biofizika. 1975 Sep-Oct;20(5):853-8.

PMID:1100
Abstract

The characteristics of light scattering of cell suspensions in norm (pH 7,2, t=20degreesC) and upon external influences (change of pH and increase of tdegree). The turbidity tauapproximatelylambda-n and n=0,2--0,3 for cells in norm. After cell damage n increases. Dependence of n correlates with the increase of some injured cells determined by eozin test. Alterations of light scattering after cell damage were connected with the increase of deposit of intercellular structure in general scattering.

摘要

细胞悬液在正常状态(pH 7.2,温度20摄氏度)以及受到外部影响(pH变化和温度升高)时的光散射特性。正常状态下细胞的浊度τ近似于λ⁻ⁿ,n = 0.2 - 0.3。细胞受损后n值增加。n的依赖性与伊红试验确定的一些受损细胞的增加相关。细胞受损后光散射的改变与一般散射中细胞间结构沉积物的增加有关。

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