McGann L E, Walterson M L, Hogg L M
Department of Pathology, University of Alberta, Edmonton, Canada.
Cytometry. 1988 Jan;9(1):33-8. doi: 10.1002/cyto.990090106.
Recent reports, indicating that under some conditions the intensity of light scattering from cells is a nonlinear function of cell volume, have led to the widespread generalization that intensity of low-angle light scattering indicates cell size. This study was performed to measure the relationships between light scattering and cell volumes in an-isotonic solutions and after a freeze-thaw stress. Cell volumes in isolated human lymphocytes, human granulocytes, and hamster fibroblasts were deliberately altered by exposure to anisotonic solutions. Boyle-vant Hoff plots of cell volume as a function of inverse osmotic pressure showed that the cells behaved as osmometers. Similar plots of right-angle and low-angle light scattering showed that the intensity of light scattering varied inversely with cell volume. In other experiments where cells were frozen without cryoprotectant at various sub zero temperatures to -25 degrees C and then thawed rapidly, cell viability decreased progressively with decreasing temperature, as did the intensity of both low-angle and right-angle light scattering, although cell volumes remained relatively constant. The intensity of both low- and high-angle light scattering varied inversely with cell volumes in hypertonic and hypotonic solutions, but cell damage induced by freezing and thawing resulted in significant reductions in the intensity of low-angle light scattering with little change in cell volume. These observations show that light scattering and cell volumes can vary independently, and they underline the need for a better understanding of the phenomenon of light scattering from living cells.
最近的报告表明，在某些条件下，细胞光散射强度是细胞体积的非线性函数，这导致了一种普遍的观点，即低角度光散射强度表明细胞大小。本研究旨在测量等渗溶液中以及冻融应激后光散射与细胞体积之间的关系。通过暴露于非等渗溶液中，故意改变分离的人淋巴细胞、人粒细胞和仓鼠成纤维细胞的细胞体积。以细胞体积作为渗透压倒数的函数绘制的玻意耳 - 范特霍夫图表明，细胞表现为渗透压计。直角和低角度光散射的类似图表明，光散射强度与细胞体积成反比。在其他实验中，细胞在各种零下温度至 -25℃下无冷冻保护剂冷冻，然后快速解冻，细胞活力随温度降低而逐渐降低，低角度和直角光散射强度也随之降低，尽管细胞体积保持相对恒定。在高渗和低渗溶液中，低角度和高角度光散射强度均与细胞体积成反比，但冻融诱导的细胞损伤导致低角度光散射强度显著降低，而细胞体积变化不大。这些观察结果表明，光散射和细胞体积可以独立变化，它们强调了更好地理解活细胞光散射现象的必要性。
