Al Musawi Mustafa S, Jaafar M S, Al-Gailani B, Ahmed Naser M, Suhaimi Fatanah M, Suardi Nursakinah
School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.
Department of Physiology, College of Medicine, Al-Mustansiriya University, Baghdad, Iraq.
Lasers Med Sci. 2017 Feb;32(2):405-411. doi: 10.1007/s10103-016-2134-1. Epub 2017 Jan 3.
Low-level laser irradiation (LLLI) has various effects on cultured human lymphocytes in vitro, but little is known about such effects in whole blood. This study investigated whether LLLI affected lymphocyte count in human whole blood in vitro. A total number of 130 blood samples were collected from apparently healthy adult patients through venipuncture into tubes containing EDTA. Each sample was divided into two equal aliquots to be used as a non-irradiated control sample and an irradiated sample. The irradiated aliquot was subjected to laser wavelengths of 405, 589, and 780 nm with different fluences of 36, 54, 72, and 90 J/cm, at a fixed irradiance of 30 mW/cm. A paired student t test was used to compare between non-irradiated and irradiated samples. The lymphocyte counts were measured using a computerized hematology analyzer and showed a significant (P < 0.02) maximum increase (1.6%) at a fluence of 72 J/cm when compared with non-irradiated samples. This increase in lymphocyte count upon irradiation was confirmed by flow cytometry. At a wavelength of 589 nm and fluence of 72 J/cm, irradiation of whole blood samples showed a significant increase in CD45 lymphocytes and natural killer (NK) (CD16, CD56) cells, but no significant changes in CD3 T lymphocytes, T-suppressor (CD3, CD8) cells, T-helper (CD3, CD4) cells, and CD19 B lymphocytes when compared with their non-irradiated counterparts. Our results clearly demonstrate that NK cell count is altered by irradiation, which ultimately affects the whole lymphocyte count significantly.
低强度激光照射（LLLI）在体外对培养的人淋巴细胞有多种影响，但对全血中的此类影响知之甚少。本研究调查了LLLI在体外是否会影响人全血中的淋巴细胞计数。通过静脉穿刺从表面健康的成年患者中采集了总共130份血样，放入含有乙二胺四乙酸（EDTA）的试管中。每个样本被分成两个相等的等分试样，分别用作未照射的对照样本和照射样本。照射的等分试样接受波长为405、589和780nm的激光照射，不同的能量密度为36、54、72和90J/cm²，固定辐照度为30mW/cm²。使用配对学生t检验比较未照射和照射样本。使用计算机化血液分析仪测量淋巴细胞计数，结果显示，与未照射样本相比，在能量密度为72J/cm²时，淋巴细胞计数显著增加（P<0.02），最大增幅为1.6%。通过流式细胞术证实了照射后淋巴细胞计数的增加。在波长为589nm、能量密度为72J/cm²时，全血样本照射后，与未照射的对应样本相比，CD45淋巴细胞和自然杀伤（NK）（CD16、CD56）细胞显著增加，但CD3 T淋巴细胞、抑制性T细胞（CD3、CD8）、辅助性T细胞（CD3、CD4）和CD19 B淋巴细胞无显著变化。我们的结果清楚地表明，照射会改变NK细胞计数，最终显著影响全淋巴细胞计数。
