Fahrenkrug S C, Freking B A, Rexroad C E, Leymaster K A, Kappes S M, Smith T P
U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA.
Mamm Genome. 2000 Oct;11(10):871-6. doi: 10.1007/s003350010150.
We used a comparative mapping approach to identify segments of conserved synteny between human Chromosome 14 (HSA14), bovine Chromosome 21 (BTA21), and the portion of ovine Chromosome 18 (OAR18) that contains the clpg locus. A bovine radiation hybrid map of the region was constructed with available Type II genetic markers and seven candidate genes to establish the comparative interval between BTA21 and HSA14. We developed polymorphic microsatellite and SNP markers associated with five candidate genes and placed them on the ovine and/or bovine genetic maps by multipoint linkage analysis. Three additional genes were mapped by virtue of their physical linkage to genetically mapped makers. Development of integrated linkage and physical maps facilitates the selection of positional candidate genes from the gene rich human map. The physically linked candidate genes PREF-1 and MEG3 map to the interval containing the clpg locus. Comparative biology suggests imprinting of MEG3 and/or the influences of PREF-1 on cellular differentiation, should be examined for their role in the parent-of-origin dependent influence of mutant clpg alleles on sheep muscle characteristics.
我们采用比较作图方法，来鉴定人类14号染色体（HSA14）、牛21号染色体（BTA21）以及绵羊18号染色体（OAR18）上包含clpg基因座的部分之间的保守同线性区段。利用现有的II型遗传标记和7个候选基因构建了该区域的牛辐射杂种图谱，以确定BTA21和HSA14之间的比较区间。我们开发了与5个候选基因相关的多态微卫星和SNP标记，并通过多点连锁分析将它们定位到绵羊和/或牛的遗传图谱上。另外3个基因通过与遗传定位标记的物理连锁进行了定位。综合连锁图谱和物理图谱的构建，有助于从基因丰富的人类图谱中选择位置候选基因。物理连锁的候选基因PREF-1和MEG3定位于包含clpg基因座的区间。比较生物学表明，应研究MEG3的印记和/或PREF-1对细胞分化的影响，以确定它们在突变clpg等位基因对绵羊肌肉特征的亲本来源依赖性影响中的作用。
