Yilmaz G, Esser P, Kociok N, Aydin P, Heimann K
Department of Ophthalmology, Baskent University School of Medicine, Ankara, Turkey.
Am J Ophthalmol. 2000 Jul;130(1):87-90. doi: 10.1016/s0002-9394(00)00398-6.
The aim of this study was to determine nitric oxide levels in the vitreous of patients with proliferative diabetic retinopathy.
Using the spectrophotometric method based on Griess reaction, we measured levels of nitrite, the stable product of nitric oxide, in the vitreous of 21 eyes of 21 patients who underwent vitrectomy for the treatment of proliferative diabetic retinopathy with tractional retinal detachment, prospectively. Three samples were excluded from the study because of blood contamination. The control group was made up of vitreous samples from 15 eyes of 15 normal cadavers and five eyes of five patients who were undergoing vitrectomy for macular hole surgery.
Nitrite levels were 0. 524 +/- 0.27 microM and 0.383 +/- 0.17 microM in the vitreous of patients with proliferative diabetic retinopathy of diabetes type I and type II, respectively. In 15 cadaver eyes and five vitreous samples from patients who underwent macular hole surgery, nitrite levels were below the detection limit (less than 0.08 microM). There was no significant difference between nitrite levels in patients with type I and type II diabetes (P =.56), whereas there was a significant difference between diabetes groups and controls (P <. 00001).
Vitreous nitric oxide levels are elevated in patients with proliferative diabetic retinopathy with tractional retinal detachment. Nitric oxide may play a role in the pathogenesis of proliferative diabetic retinopathy.
本研究旨在测定增殖性糖尿病视网膜病变患者玻璃体中的一氧化氮水平。
我们采用基于格里斯反应的分光光度法，前瞻性地测量了21例因牵引性视网膜脱离接受玻璃体切除术治疗增殖性糖尿病视网膜病变患者的21只眼中一氧化氮的稳定产物亚硝酸盐水平。由于血液污染，3个样本被排除在研究之外。对照组由15例正常尸体的15只眼和5例因黄斑裂孔手术接受玻璃体切除术患者的5只眼的玻璃体样本组成。
I型和II型糖尿病增殖性糖尿病视网膜病变患者玻璃体中的亚硝酸盐水平分别为0.524±0.27微摩尔/升和0.383±0.17微摩尔/升。在15只尸体眼和5例接受黄斑裂孔手术患者的玻璃体样本中，亚硝酸盐水平低于检测限（低于0.08微摩尔/升）。I型和II型糖尿病患者的亚硝酸盐水平之间无显著差异（P = 0.56），而糖尿病组与对照组之间存在显著差异（P < 0.00001）。
伴有牵引性视网膜脱离的增殖性糖尿病视网膜病变患者玻璃体中的一氧化氮水平升高。一氧化氮可能在增殖性糖尿病视网膜病变的发病机制中起作用。
