Distribution of cytophilic and anti-macrophage antibody on the macrophage surface.

The binding of cytophilic antibody to the macrophage surface results in patterns of distribution similar to those obtained when specific anti-macrophage antibody is used. These patterns are (a) diffuse circumferential binding at 4 degrees C, and (b) capping at 37 degrees C. The non-random distribution of the cytophilic antibody suggests that it binds to certain well-defined sites on the macrophage membrane.