Bondi A M, Gabrielli M G, Accili D, Sabbieti M G, Menghi G
Department of Comparative Morphological and Biochemical Sciences, University of Camerino, Italy.
Histol Histopathol. 2000 Oct;15(4):1119-25. doi: 10.14670/HH-15.1119.
A confocal analysis was performed on the quail (Coturnix coturnix japonica) lingual salivary glands where the carbohydrate chains were studied by lectin histochemistry. For this purpose, appropriate FITC- and TRITC-conjugates were used for double binding also accomplished with sialidase digestion. The glycosidic components of the quail lingual salivary glands were found to be heterogeneously distributed on the different secretory structures as well as on the single secretory elements of each adenomere. The rostral portion of the anterior lingual gland was found to only secrete neutral glycocomponents, characterized by terminal beta-galactose, N-acetylgalactosamine and fucose residues in contrast to the caudal portion that was shown to be extremely heterogeneous and to produce sialylated glycoconjugates characterized by the terminal sequences sialic acid-beta-galactose-N-acetylgalactosamine, sialic acid-beta-galactose-N-acetylglucosamine, and sialic acid-alpha-N-acetylgalactosamine partly codistributed within secretory adenomeres. The posterior lingual gland was observed to be the major contributor to the secretion of salivary mucins containing sialoglycoconjugates with terminal sialic acid residues linked to beta-galactose-N-acetylgalactosamine or alpha-N-acetylgalactosamine often located in distinct secretory elements.
对鹌鹑（日本鹌鹑）舌唾液腺进行了共聚焦分析，通过凝集素组织化学研究碳水化合物链。为此，使用了合适的异硫氰酸荧光素（FITC）和四甲基异硫氰酸罗丹明（TRITC）缀合物进行双重结合，同时也进行了唾液酸酶消化。发现鹌鹑舌唾液腺的糖苷成分在不同的分泌结构以及每个腺小叶的单个分泌元件上分布不均。发现舌前腺的前部仅分泌中性糖成分，其特征为末端β-半乳糖、N-乙酰半乳糖胺和岩藻糖残基，而尾部则显示出高度异质性，并产生唾液酸化糖缀合物，其特征为末端序列唾液酸-β-半乳糖-N-乙酰半乳糖胺、唾液酸-β-半乳糖-N-乙酰葡糖胺和唾液酸-α-N-乙酰半乳糖胺，部分共分布于分泌腺小叶内。观察到舌后腺是唾液粘蛋白分泌的主要贡献者，这些粘蛋白含有唾液酸化糖缀合物，其末端唾液酸残基与β-半乳糖-N-乙酰半乳糖胺或α-N-乙酰半乳糖胺相连，通常位于不同的分泌元件中。
