Gargiulo A M, Pedini V, Ceccarelli P
Istituto di Anatomia Normale degli Animali Domestici, Università degli Studi di Perugia, Italy.
Arch Oral Biol. 1993 Oct;38(10):881-4. doi: 10.1016/0003-9969(93)90097-6.
Bovine lingual glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates was obtained by means of a battery of peroxidase-conjugated lectins with affinity for specific terminal sugars. Sialidase procedures followed by lectin staining were also used to visualize the sugar sequences. Stored secretions in mucous acinar cells contained fucose, N-acetylglucosamine, alpha and beta-N-acetylgalactosamine as terminal sugar residues and beta-galactose as penultimate sugar in a heterogeneous distribution. Demilunar cells failed to react with any of the lectins examined except that of Dolichos biflorus.
牛舌腺由半月形覆盖的黏液性腺泡组成。通过一系列对特定末端糖具有亲和力的过氧化物酶偶联凝集素,获得了其分泌性糖缀合物的化学结构信息。唾液酸酶处理后进行凝集素染色也用于观察糖序列。黏液性腺泡细胞中储存的分泌物含有岩藻糖、N-乙酰葡糖胺、α和β-N-乙酰半乳糖胺作为末端糖残基,以及β-半乳糖作为倒数第二个糖,分布不均一。半月形细胞除了与双花扁豆凝集素外,与所检测的任何凝集素均无反应。