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用于研究日本鹌鹑(Coturnix coturnix japonica)舌唾液腺中糖缀合物的基础组织化学和凝集素组织化学。

Basic and lectin histochemistry for studying glycoconjugates in the lingual salivary glands of the Japanese quail (Coturnix coturnix japonica).

作者信息

Menghi G, Scocco P, Ceccarelli P

机构信息

Dipartimento di Biologia M.C.A., Università di Camerino, Italy.

出版信息

Arch Oral Biol. 1993 Aug;38(8):649-55. doi: 10.1016/0003-9969(93)90003-5.

DOI:10.1016/0003-9969(93)90003-5
PMID:8215987
Abstract

Traditional non-lectin staining methods and lectin histochemistry combined with specific exoglycosidase digestion were used to investigate the histological structure of these glands and to visualize the occurrence and composition of salivary glycoconjugates. The quail lingual glands comprise the anterior lingual gland, with rostral and caudal portions that differ in morphology and histochemical staining, and the posterior lingual gland devoid of regional differences. Carbohydrate histochemistry further differentiated the secretory structures by revealing a heterogeneous cell population of tubules within the rostral and caudal portions of the anterior gland, and a rather homogeneous staining of the tubuloalveolar elements in the rostral portion of the anterior lingual gland and in the posterior gland. Tubuloalveolar secretory cells mainly produced sulphate-containing molecules. In addition, sialoglycoconjugates were present in both anterior and posterior lingual glands, whereas fucoglycoconjugates were visualized only in the anterior gland.

摘要

采用传统的非凝集素染色方法以及结合特定外切糖苷酶消化的凝集素组织化学方法,来研究这些腺体的组织结构,并观察唾液糖缀合物的存在情况和组成。鹌鹑舌腺包括前舌腺,其头侧部和尾侧部在形态和组织化学染色上有所不同,以及没有区域差异的后舌腺。碳水化合物组织化学通过揭示前腺头侧部和尾侧部内不同的小管细胞群,以及前舌腺头侧部和后腺中管泡状结构相当均匀的染色,进一步区分了分泌结构。管泡状分泌细胞主要产生含硫酸盐的分子。此外,唾液酸糖缀合物在前舌腺和后舌腺中均有存在,而岩藻糖糖缀合物仅在前腺中可见。

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