用于冷冻保存和回输的人骨髓处理
The processing of human bone marrow for cryopreservation and reinfusion.
作者信息
Weiner R S, Tobias J S, Yankee R A
出版信息
Biomedicine. 1976;24(4):226-31.
Bone marrow was aspirated from 22 patients with solid tumors. A median of 1.2 x 10(10) net bone marrow cells were obtained during a 1 hour 45 minute operative procedure. Three methods were used to concentrate the bone marrow for cryopreservation. The greatest concentration of mononuclear cells was obtained by the Haemonetics pheresis procedure. Bone marrow was cryopreserved in 10 % DMSO in volumes ranging from 150 to 700 ml. The thawed marrow was further processed to remove both DMSO and free hemoglobin prior to reinfusion. Cell clumping was observed in the first 6 of the 10 marrows thawed. Acidification of the thawed marrow to pH 6.65 was found to retard cell clumping. The immediate recovery of cells frozen was 66 % with a viability of 50 %. The mean number of viable mononuclear bone marrow cells per kilogram available for reinfusion was therefore 5.5 x 10(7).
从22例实体瘤患者中抽取骨髓。在1小时45分钟的手术过程中，平均获得1.2×10¹⁰个净骨髓细胞。采用三种方法浓缩骨髓用于冷冻保存。通过血细胞分离程序获得的单核细胞浓度最高。骨髓在含10%二甲亚砜的溶液中冷冻保存，体积为150至700毫升。解冻后的骨髓在回输前进一步处理以去除二甲亚砜和游离血红蛋白。在解冻的10份骨髓中的前6份中观察到细胞聚集。发现将解冻后的骨髓酸化至pH 6.65可延缓细胞聚集。冷冻细胞的即时回收率为66%，活力为50%。因此，每千克可用于回输的存活单核骨髓细胞的平均数量为5.5×10⁷。
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