骨髓处理与冷冻保存。
Bone marrow processing and cryopreservation.
作者信息
Linch D C, Knott L J, Patterson K G, Cowan D A, Harper P G
出版信息
J Clin Pathol. 1982 Feb;35(2):186-90. doi: 10.1136/jcp.35.2.186.
Abstract
Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30 cell separator and an Aminco Celltrifuge. The best results were achieved using the paediatric pheresis set on the Hemonetics model 30. Marrow was frozen in 120 ml aliquots in a programmed freezer with rapid cooling of the freezing chamber during the phase change from the liquid to the solid state. After freeze-thawing the average nucleated cell recovery was approximately 50% and the progenitor cell recovery 80%.
摘要
在冷冻保存前，对三种不同的浓缩骨髓祖细胞的封闭程序进行了比较。这三种程序分别是手动双离心法、Hemonetics 30细胞分离器法和Aminco细胞离心机法。使用Hemonetics 30型儿科血液成分单采设备获得了最佳结果。骨髓以120毫升分装在程序降温冷冻机中冷冻，在从液态到固态的相变过程中对冷冻室进行快速冷却。冻融后，平均有核细胞回收率约为50%，祖细胞回收率为80%。
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