Jennings J J, Baltz M, Rittenberg M B
J Immunol. 1975 Nov;115(5):1432-7.
A primary immune response was obtained in vitro to trinitrophenylated bacteriophate T4 (TNP-T4) by using normal BALB/c spleen cells challenged in vitro. The primary response was limited to anti-TNP; we failed to find evidence of a primary anti-T4 response in spleen cell cultures challenged with either TNP-T4 or native T4. In vivo priming with the carrier T4 failed to enhance the subsequent in vitro response to TNP-T4 although such priming sometimes caused suppression of the anti-hapten response. The addition of excess free carrier to spleen cell cultures challenged with TNP-T4 failed to suppress the anti-tnp response. Spleen cells treated with anti-theta plus complement were unaffected in their ability to respond to TNP-T4. We conclude that TNP-T4 should be classified among the thymus independent antigens.
通过体外刺激正常BALB/c脾细胞，在体外获得了针对三硝基苯化噬菌体T4（TNP-T4）的初次免疫应答。初次应答仅限于抗TNP；在用TNP-T4或天然T4刺激的脾细胞培养物中，我们未能找到初次抗T4应答的证据。用载体T4进行体内预刺激未能增强随后对TNP-T4的体外应答，尽管这种预刺激有时会导致抗半抗原应答受到抑制。向用TNP-T4刺激的脾细胞培养物中添加过量的游离载体未能抑制抗TNP应答。用抗θ加补体处理的脾细胞对TNP-T4的应答能力未受影响。我们得出结论，TNP-T4应归类于胸腺非依赖性抗原。
