大肠杆菌K来源的限制性内切核酸酶识别DNA的多个步骤。
Multiple steps in DNA recognition by restriction endonuclease from E. coli K.
作者信息
Yuan R, Bickle T A, Ebbers W, Brack C
出版信息
Nature. 1975 Aug 14;256(5518):556-60. doi: 10.1038/256556a0.
DOI:10.1038/256556a0
PMID:1101070
Abstract
The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.
摘要
来自大肠杆菌K的限制性内切核酸酶的活化形式识别DNA的过程涉及三种可通过实验区分的酶-DNA复合物。它们是：在非特异性位点形成的初始复合物；涉及宿主特异性位点的识别复合物；以及依赖于ATP存在的切割复合物。
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