Steenkamp E T, Wingfield B D, Coutinho T A, Zeller K A, Wingfield M J, Marasas W F, Leslie J F
Tree Pathology Co-Operative Programme, Forestry and Agricultural Biotechnology Institute, Departments of Genetics, Microbiology, and Plant Pathology, University of Pretoria, Pretoria 0002, South Africa.
Appl Environ Microbiol. 2000 Oct;66(10):4378-82. doi: 10.1128/AEM.66.10.4378-4382.2000.
All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphs MAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an approximately 800-bp fragment from MAT-2 or an approximately 200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, and Fusarium culmorum, which have, or are expected to have, Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and other Fusarium species.
藤仓赤霉菌复合种中所有具有有性繁殖能力的菌株都是异宗配合的，其个体交配型由广泛保守的子囊菌特异性基因座MAT-1和MAT-2决定。我们对所有八个交配群体的两个等位基因进行了测序，开发了一种多重PCR技术来区分这些特异性基因座，并用来自所有八个生物种以及该复合种中另外22个物种或系统发育谱系的代表性菌株对其进行了测试。在大多数情况下，要么扩增出MAT-2的约800 bp片段，要么扩增出MAT-1的约200 bp片段。在串珠镰孢菌（轮枝镰孢菌）的杂交中，扩增片段与由有性杂交可育性定义的交配型共分离。这两对引物均未从禾谷镰孢菌、拟禾谷镰孢菌和燕麦镰孢菌等镰孢菌物种中扩增出片段，这些物种具有或预期具有赤霉菌有性阶段，但被认为与藤仓赤霉菌复合种中的物种亲缘关系相对较远。我们的结果表明，MAT等位基因序列是这些及其他镰孢菌物种系统发育相关性的有用指标。
