Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, Pernambuco, Brazil.
Mycologia. 2012 Nov-Dec;104(6):1408-19. doi: 10.3852/12-052. Epub 2012 Jun 6.
Fusarium tupiense, the main causal agent of mango malformation in Brazil, is described through a combination of morphological, biological and molecular markers. This new species belongs to the Gibberella fujikuroi species complex (GFSC) and has an anamorph morphologically similar to Fusarium mangiferae and F. sterilihyphosum. F. tupiense can be differentiated from other species in the G. fujikuroi species complex on the basis of sexual crosses, amplified fragment length polymorphism (AFLP) markers and partial sequences of the tef1 and tub2 genes. Female fertility for field isolates of F. tupiense appears to be low. PCR with primers specific for the mating type (MAT) alleles and sexual crosses identified this species as heterothallic with two idiomorphs. Female-fertile tester strains were developed for the identification of field strains of this species through sexual crosses.
藤仓镰刀菌是巴西芒果畸形病的主要致病因子，通过形态学、生物学和分子标记的综合手段进行了描述。该新种属于藤仓镰刀菌种复合体（GFSC），其无性型在形态上与芒果镰刀菌和 F. sterilihyphosum 相似。基于有性杂交、扩增片段长度多态性（AFLP）标记和 tef1 和 tub2 基因的部分序列，F. tupiense 可与 GFSC 中的其他种相区分。藤仓镰刀菌田间分离物的雌性育性似乎较低。针对交配型（MAT）等位基因的 PCR 和有性杂交鉴定该种为异宗配合，具有两个异型。通过有性杂交，为鉴定该种的田间菌株，开发了用于鉴定该种的雌性可育测试菌株。
