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Fusarium subglutinans f. sp. Pini represents a distinct mating population in the gibberella fujikuroi species complex.亚粘团镰孢菌松专化型在藤仓赤霉复合种中代表一个独特的交配群体。
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藤仓赤霉菌复合种交配型术语的分子标准化

Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex.

作者信息

Kerényi Z, Zeller K, Hornok L, Leslie J F

机构信息

Agricultural Biotechnology Center, Gödöllo, Hungary.

出版信息

Appl Environ Microbiol. 1999 Sep;65(9):4071-6. doi: 10.1128/AEM.65.9.4071-4076.1999.

DOI:10.1128/AEM.65.9.4071-4076.1999
PMID:10473418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC99743/
Abstract

Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and is usually identified following sexual crosses with standard, female-fertile tester isolates. The mating type alleles have been arbitrarily designated "+" and "-" within each biological species, and the nomenclature is tied to the standard tester strains. We developed a pair of PCR primers that can be used to amplify a unique fragment of one of the mating type alleles (MAT-2) from at least seven of the biological species in this species complex. Based on the amplification pattern, we propose a replacement for the existing, arbitrary +/- terminology that is presently in use. The new terminology is based on DNA sequence similarities between the mating type allele fragments from the biological species of the G. fujikuroi species complex and the corresponding fragments from other filamentous ascomycetes.

摘要

藤仓赤霉菌复合种的交配型由一个具有两个等位基因的单一位点控制，通常通过与标准的、可育雌性测试菌株进行有性杂交来鉴定。在每个生物物种中，交配型等位基因被任意指定为“+”和“-”，并且该命名法与标准测试菌株相关。我们开发了一对PCR引物，可用于从该复合种中的至少七个生物物种中扩增交配型等位基因之一（MAT-2）的独特片段。基于扩增模式，我们提议替换目前使用的现有的任意+/-术语。新术语基于藤仓赤霉菌复合种生物物种的交配型等位基因片段与其他丝状子囊菌的相应片段之间的DNA序列相似性。