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一种用于双孢蘑菇农杆菌介导高效转化的子实体组织方法。

A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus.

作者信息

Chen X, Stone M, Schlagnhaufer C, Romaine C P

机构信息

Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

出版信息

Appl Environ Microbiol. 2000 Oct;66(10):4510-3. doi: 10.1128/AEM.66.10.4510-4513.2000.

DOI:10.1128/AEM.66.10.4510-4513.2000
PMID:11010906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92332/
Abstract

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.

摘要

我们描述了一种改良的农杆菌介导法，用于双孢蘑菇的高效转化。该方法的显著特点包括农杆菌与子实体菌褶组织的共培养以及使用带有同源启动子的载体。此方法为这种具有重要商业价值的蘑菇物种的基因操作提供了新的前景。

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本文引用的文献

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Agrobacterium tumefaciens-mediated transformation of filamentous fungi.根癌农杆菌介导的丝状真菌转化
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Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.要使转基因拟南芥植株得到明亮标记，需要去除一个隐蔽内含子并进行绿色荧光蛋白的亚细胞定位。
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A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.一种经改造以在哺乳动物细胞中实现高水平表达的“人源化”绿色荧光蛋白cDNA。
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Curr Genet. 1996 Jul 31;30(2):166-73. doi: 10.1007/s002940050116.
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Mol Gen Genet. 1996 Feb 25;250(3):252-8. doi: 10.1007/BF02174382.