Spellig T, Bottin A, Kahmann R
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Mol Gen Genet. 1996 Oct 16;252(5):503-9. doi: 10.1007/BF02172396.
Pathogenic development of Ustilago maydis, the causative agent of corn smut disease, is a multistep process. Compatible yeast-like cells fuse and this generates the infectious dikaryon which grows filamentously. Having entered the plant the dikaryon induces tumors in its host in which massive proliferation of fungal material, karyogamy and spore formation occur. In order to follow fungal development from the initial steps to the final stage we have expressed the green fluorescent protein (GFP) from Aequorea victoria as a vital marker in U. maydis and demonstrate that GFP-tagged strains can be used to study host-pathogen interactions in vivo.
玉米丝黑穗病的病原体玉米黑粉菌的致病发育是一个多步骤过程。兼容的酵母样细胞融合，产生传染性双核体，该双核体呈丝状生长。进入植物后，双核体在其宿主中诱导肿瘤形成，在肿瘤中发生真菌物质的大量增殖、核配和孢子形成。为了追踪真菌从初始阶段到最终阶段的发育过程，我们表达了来自维多利亚水母的绿色荧光蛋白（GFP）作为玉米黑粉菌的一种活体标记，并证明带有GFP标签的菌株可用于研究体内宿主-病原体相互作用。
