Clermont O, Bonacorsi S, Bingen E
Laboratoire d'études de génétique bactérienne dans les infections de l'enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France.
Appl Environ Microbiol. 2000 Oct;66(10):4555-8. doi: 10.1128/AEM.66.10.4555-4558.2000.
Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
系统发育分析表明，大肠杆菌由四个主要的系统发育群（A、B1、B2和D）组成，且致病性肠外菌株主要属于B2和D群。实际上，系统发育群可通过多位点酶电泳或核糖体分型来确定，这两种方法都是复杂且耗时的技术。我们描述了一种基于三重PCR的简单快速的系统发育分组技术。该方法使用两个基因（chuA和yjaA）和一个匿名DNA片段的组合，对230株菌株进行了测试，结果显示与参考方法具有良好的相关性。
