Kholik Kholik, Sukri Akhmad, Priscilia Riwu Katty Hendriana, Ayu Ieke Wulan, Dewi Ika Nurani
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia.
Department of Biology Education, Faculty of Science and Engineering, Universitas Pendidikan Mandalika, Mataram, Indonesia.
Vet Res Forum. 2025;16(6):325-330. doi: 10.30466/vrf.2024.2039823.4421. Epub 2025 Jun 15.
The transmission of () containing virulent genes from animals to humans and the environment poses significant public health challenges. This study aimed to detect the virulence factor of the () in isolated from the feces of apparently healthy horses in the island of Sumbawa, Indonesia. The study utilized 52 fecal samples from a total horse population of 283, calculated using the disease detection formula. Fresh feces were collected immediately after excretion and placed in buffered peptone water for subsequent analysis. The samples were then isolated on eosin methylene blue media and identified using biochemical tests. Identified strains were further examined for detecting the gene using polymerase chain reaction techniques. The was successfully isolated and identified in 11 (21.15%) of the 52 collected fecal samples. Polymerase chain reaction analysis detected the gene in 8 (15.38%) isolates at 279 bp on gel electrophoresis. The close interaction between horses and humans in the island of Sumbawa, Indonesia, may facilitate the spread of . Thus, surveillance is needed to employ a One Health approach to monitor strains encoding the gene and other virulence factors to control their dissemination.
携带毒力基因的（）从动物传播到人类和环境对公共卫生构成了重大挑战。本研究旨在检测从印度尼西亚松巴哇岛表面健康马匹粪便中分离出的（）的毒力因子。该研究使用疾病检测公式计算得出，从总共283匹马中选取了52份粪便样本。粪便排出后立即收集新鲜粪便，并置于缓冲蛋白胨水中用于后续分析。然后将样本在伊红美蓝培养基上分离，并通过生化试验进行鉴定。使用聚合酶链反应技术对鉴定出的（）菌株进一步检测（）基因。在收集的52份粪便样本中，成功分离并鉴定出11株（21.15%）（）。聚合酶链反应分析在凝胶电泳上于279 bp处检测到8株（15.38%）（）分离株中的（）基因。在印度尼西亚松巴哇岛，马匹与人类之间的密切互动可能会促进（）的传播。因此，需要采用“同一个健康”方法进行监测，以监控编码（）基因和其他毒力因子的（）菌株，从而控制它们的传播。
