一种研究tRNA三维结构的化学方法——用与“特殊位点”共价结合的试剂进行烷基化
A chemical approach to studies of the three-dimensional structure of tRNA - alkylation with a reagent covalently bound to a "peculiar site'.
作者信息
Grachev M A, Rivkin M I
出版信息
Nucleic Acids Res. 1975 Aug;2(8):1237-60. doi: 10.1093/nar/2.8.1237.
Abstract
Yeast valine tRNA1 was chemically modified with chlorambucil N-hydroxysuccinimide ester. tthe reagent was attached covalently to the valine residue of valyl-tRNA1Val under the conditions which prevented tRNA from alkylation. Chlorambucilyl-valyl-tRNA1Val thus obtained was separated from excess reagent and incubated in an aqueous solution at neutral pH in the presence of Mg++ions. Highly efficient intramolecular self-alkylation of chlorambucilyl-valyl-tRNA1Val took place. The chlorambucil residue bound covalently to the amino group of the valine residue of tRNA1Val alkylates the 5'-terminal phosphate group of the molecule, and its 3'-terminal sequence -A-C-C-A.
摘要
酵母缬氨酸tRNA1用苯丁酸氮芥N - 羟基琥珀酰亚胺酯进行化学修饰。该试剂在防止tRNA烷基化的条件下共价连接到缬氨酰 - tRNA1Val的缬氨酸残基上。由此得到的苯丁酸氮芥酰 - 缬氨酰 - tRNA1Val与过量试剂分离，并在中性pH的水溶液中、Mg++离子存在下孵育。苯丁酸氮芥酰 - 缬氨酰 - tRNA1Val发生了高效的分子内自烷基化。与tRNA1Val的缬氨酸残基的氨基共价结合的苯丁酸氮芥残基使该分子的5'-末端磷酸基团及其3'-末端序列 -A-C-C-A烷基化。
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A chemical approach to studies of the three-dimensional structure of tRNA - alkylation with a reagent covalently bound to a "peculiar site'.一种研究tRNA三维结构的化学方法——用与“特殊位点”共价结合的试剂进行烷基化
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本文引用的文献
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ON THE STABILITY OF AMINOACYL-S-RNA TO NUCLEOPHILIC CATALYSIS.氨基酰 - S - RNA对亲核催化作用的稳定性研究
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7
A specific chemical chain scission of tRNA at 7-methylguanosine.转运核糖核酸（tRNA）在7-甲基鸟苷处的特定化学链断裂。
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Tetrahedron Lett. 1967 Sep;37:3557-62. doi: 10.1016/s0040-4039(01)89794-x.
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[A preparative procedure for the isolation of enriched preparations of aminoacyl-tRNA-synthetases from baker's yeast].[一种从面包酵母中分离富含氨酰 - tRNA合成酶制剂的制备方法]
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