Sanada N, Sanada Y
Kyuritsu Shoji Laboratories, Ibaraki, Japan.
J Vet Med B Infect Dis Vet Public Health. 2000 Aug;47(6):441-3. doi: 10.1046/j.1439-0450.2000.00360.x.
The erythrocytes of various species were tested in psittacine beak and feather disease (PBFD) virus haemagglutination (HA) and haemagglutination inhibition assays to determine which are suitable for use in these assays. HA activity was observed for erythrocytes of the salmon-crested cockatoo, the sulphur-crested cockatoo, the umbrella cockatoo, the goffin's cockatoo and the cockatiel, with differences amongst individuals within species, but not for erythrocytes of humans, the pig, the guinea pig, the chicken, the goose, the rose-ringed parakeet or the budgerigar. Anti-PBFD virus rabbit sera inhibited the virus-induced agglutination of erythrocytes, confirming the specificity of HA activity. This suggests that selection of suitable psittacine species as well as suitable individuals within a species is necessary when obtaining erythrocytes for the PBFD virus HA assay.
在鹦鹉喙羽病（PBFD）病毒血凝（HA）和血凝抑制试验中对各种物种的红细胞进行了测试，以确定哪些适用于这些试验。鲑色凤头鹦鹉、硫冠凤头鹦鹉、雨伞凤头鹦鹉、戈芬氏凤头鹦鹉和鸡尾鹦鹉的红细胞表现出血凝活性，同一物种内个体之间存在差异，但人类、猪、豚鼠、鸡、鹅、环颈鹦鹉或虎皮鹦鹉的红细胞则未表现出血凝活性。抗PBFD病毒兔血清可抑制病毒诱导的红细胞凝集，证实了血凝活性的特异性。这表明，在为PBFD病毒血凝试验获取红细胞时，选择合适的鹦鹉物种以及同一物种内合适的个体是必要的。
