Ritchie B W, Niagro F D, Latimer K S, Steffens W L, Pesti D, Lukert P D
Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Am J Vet Res. 1991 Nov;52(11):1810-5.
Conditions for psittacine beak and feather disease (PBFD) virus hemagglutination and hemagglutination-inhibition (HI) test reactions are defined. The PBFD virus was found to hemagglutinate cockatoo and some guinea pig erythrocytes. The HI test was used to assay serum antibody titer in birds with active PBFD virus infections and in others that had been exposed to diseased birds. On the basis of HI antibody titers in psittacine birds that had been exposed to PBFD virus, but remained clinically normal, we suggest that some birds exposed to the virus are able to mount an effective immune response. Birds with active PBFD virus infections had lower antibody values than did birds that had been exposed to the virus, but remained clinically normal. On the basis of these findings, the ability to develop a suitable HI antibody response may be crucial in determining the disease status of susceptible birds exposed to the PBFD virus. If HI antibodies are found to have neutralizing activity, then the fact that a high HI titer was induced in birds inoculated with purified PBFD virus might suggest that an immunization program would be effective in preventing PBFD virus infections.
定义了鹦鹉喙羽病（PBFD）病毒血凝和血凝抑制（HI）试验反应的条件。发现PBFD病毒能凝集凤头鹦鹉和一些豚鼠的红细胞。HI试验用于检测感染活跃PBFD病毒的鸟类以及接触过患病鸟类的其他鸟类的血清抗体滴度。根据接触过PBFD病毒但临床仍正常的鹦鹉类鸟类的HI抗体滴度，我们认为一些接触该病毒的鸟类能够产生有效的免疫反应。感染活跃PBFD病毒的鸟类的抗体值低于接触过该病毒但临床仍正常的鸟类。基于这些发现，产生合适的HI抗体反应的能力可能对确定接触PBFD病毒的易感鸟类的疾病状态至关重要。如果发现HI抗体具有中和活性，那么在用纯化的PBFD病毒接种的鸟类中诱导出高HI滴度这一事实可能表明免疫程序在预防PBFD病毒感染方面将是有效的。
