Høst B, Schumacher H, Prag J, Arpi M
Department of Clinical Microbiology, Viborg Hospital, Denmark.
Eur J Clin Microbiol Infect Dis. 2000 Aug;19(8):608-11. doi: 10.1007/s100960000324.
According to the literature, Kingella kingae may be an underdiagnosed cause of joint and bone infections in children. The use of the Bactec blood culture system for culture of joint fluids has dramatically improved the isolation of this fastidious bacterium. The aim of this study was to test the recovery rate and detection time of four commercial blood culture systems: three different BacT/Alert (Organon Teknika, USA) bottles and one Bactec (Becton Dickinson Microbiology Systems, USA) bottle, all inoculated with Kingella kingae strains mixed with pooled synovial fluids. For each strain the same inoculum and volume of synovial fluid was distributed into each of the four bottles. All 24 strains tested grew in the BacT/Alert Aerobic (100%) and the BacT/Alert Pedi-BacT (100%) bottles. Twenty-one strains grew in the BacT/Alert FAN aerobic (88%) bottle, and 15 strains grew in the Bactec Plus Aerobic F (63%) bottle, in both systems within 12 days (P<0.01). The Kingella kingae strains were first detected in the BacT/Alert Pedi-BacT bottles (P<0.001). The results were reproducible. The BacT/Alert blood culture bottles were superior to previously described blood culture systems in isolating Kingella kingae from synovial fluid, even with small inoculums and small volumes of synovial fluid.
根据文献记载，金氏金杆菌可能是儿童关节和骨感染中一种未被充分诊断的病因。使用Bactec血培养系统对关节液进行培养，极大地提高了这种苛求菌的分离率。本研究的目的是测试四种商业血培养系统的回收率和检测时间：三种不同的BacT/Alert（美国Organon Teknika公司）瓶和一种Bactec（美国Becton Dickinson微生物系统公司）瓶，所有瓶子均接种了与混合滑膜液混合的金氏金杆菌菌株。对于每种菌株，将相同接种量和体积的滑膜液分别注入四个瓶子中。所有测试的24株菌株均在BacT/Alert需氧瓶（100%）和BacT/Alert儿科需氧瓶（100%）中生长。21株菌株在BacT/Alert FAN需氧瓶（88%）中生长，15株菌株在Bactec Plus需氧F瓶（63%）中生长，两种系统均在12天内生长（P<0.01）。金氏金杆菌菌株首先在BacT/Alert儿科需氧瓶中被检测到（P<0.001）。结果具有可重复性。BacT/Alert血培养瓶在从滑膜液中分离金氏金杆菌方面优于先前描述的血培养系统，即使接种量小和滑膜液体积小。
