Tardieu M, Crchova V, Daguillard F
Ann Immunol (Paris). 1975 Apr;126(3):281-9.
In previous studies we have isolated by density gradient separation a population of relatively dense T lymphocytes capable of inhibiting the mitogenic response to concanavalin A (ConA) and phytohaemagglutinin. In this study we compared two current methods of cell separation for their relative yield in T lymphocytes able or not to respond to Con A. The present results show that cells passed through nylon wool columns--in contrast to those obtained by removal of lymphocytes which bind erythrocyte-antibody-complement complex-partially lose their ability to respond to ConA. The response lost by passage through nylon wool columns can be restored by centrifugation of the T cell suspension on a gradient of "Ficoll-Hypaque". This suggests that methods of T cells purification using density gradient separation may remove the more dense suppressor cells.
在先前的研究中，我们通过密度梯度分离法分离出了一群相对致密的T淋巴细胞，它们能够抑制对刀豆蛋白A（ConA）和植物血凝素的促有丝分裂反应。在本研究中，我们比较了两种当前的细胞分离方法，看它们在能够或不能对Con A作出反应的T淋巴细胞中的相对产量。目前的结果表明，通过尼龙毛柱的细胞——与通过去除结合红细胞-抗体-补体复合物的淋巴细胞获得的细胞相反——部分丧失了对ConA作出反应的能力。通过尼龙毛柱后丧失的反应可以通过在“Ficoll-Hypaque”梯度上离心T细胞悬液来恢复。这表明使用密度梯度分离的T细胞纯化方法可能会去除密度更大的抑制细胞。
