Banks K L, Greenlee A
Am J Vet Res. 1981 Sep;42(9):1651-4.
Various cell populations of equine mononuclear leukocytes were identified and isolated. Mononuclear leukocytes were concentrated by isopyknic centrifugation, using a solution of Ficoll and Hypaque. Three additional techniques were explored to separate monocytes from lymphocytes, and 3 methods were used to separate lymphocyte types. Cytochemical techniques for the detection of nonspecific esterase readily distinguished equine monocytes from lymphocytes. Peripheral blood lymphocytes were separated into at least 2 populations. One population had surface traits identical to thymocytes [ie, they readily bound peanut agglutinin, but lacked receptors for complement or immunoglobulin (Ig) and did not have surface Ig, as detected by immunohistochemical techniques]. This population could be isolated, using nylon-wool columns, or by depletion of complement- and Ig-binding cells during centrifugation. The other class of lymphocytes had equine complement receptors, Ig receptors, and detectable surface Ig, but was not bound by peanut agglutinin. Using rosetting techniques followed by centrifugation, this latter population was enriched. These studies provided means of isolating and detecting equine monocytes, B lymphocytes, and T lymphocytes.
已识别并分离出了马单核白细胞的各种细胞群体。使用Ficoll和Hypaque溶液通过等密度离心法浓缩单核白细胞。探索了另外三种技术来从淋巴细胞中分离单核细胞，并使用了三种方法来分离淋巴细胞类型。用于检测非特异性酯酶的细胞化学技术很容易区分马单核细胞和淋巴细胞。外周血淋巴细胞至少可分为两个群体。其中一个群体具有与胸腺细胞相同的表面特征[即，它们易于结合花生凝集素，但缺乏补体或免疫球蛋白（Ig）受体，且通过免疫组织化学技术检测没有表面Ig]。使用尼龙毛柱，或在离心过程中去除补体结合细胞和Ig结合细胞，可分离出该群体。另一类淋巴细胞具有马补体受体、Ig受体和可检测到的表面Ig，但不与花生凝集素结合。通过离心后的玫瑰花结技术，富集了后一类群体。这些研究提供了分离和检测马单核细胞、B淋巴细胞和T淋巴细胞的方法。
