Nair K G, Shalia K K, Ashavaid T F, Dalal J J
Research Laboratories, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
J Clin Lab Anal. 2003;17(5):141-6. doi: 10.1002/jcla.10084.
Gene encoding components of the renin angiotensin system (RAS) have been implicated with the increased risk of cardiovascular disease (CVD). Two variants of the angiotensinogen (AGT) gene, M235T and T174M, have been shown to be associated with increased risk of hypertension. In the present study, we examined the association of these two polymorphisms and their synergistic interaction with the angiotensin I-converting enzyme (ACE) deletion homozygote genotype (D/D) on subjects with coronary heart disease (CHD) and hypertension. We studied 131 healthy individuals, 141 angiographically verified CHD patients, and 159 hypertensive subjects. The identification of the ACE and AGT gene polymorphisms was carried out using a PCR-based restriction endonuclease digestion method. There was no significant difference in the distribution of the M235T and T174M variants between the two test groups and the control group. Association was also not seen when analysis was carried out in patients when subgrouped according to the extent of the severity of the disease. In addition, the risk was not restricted to subjects carrying the D allele of the ACE gene and T235T of AGT. M235T and T174M variants do not contribute to the increased risk of CHD or hypertension in the Indian population.
肾素血管紧张素系统（RAS）的基因编码成分与心血管疾病（CVD）风险增加有关。血管紧张素原（AGT）基因的两个变体，M235T和T174M，已被证明与高血压风险增加有关。在本研究中，我们研究了这两种多态性及其与血管紧张素I转换酶（ACE）缺失纯合子基因型（D/D）在冠心病（CHD）和高血压患者中的协同相互作用。我们研究了131名健康个体、141名经血管造影证实的CHD患者和159名高血压患者。采用基于聚合酶链反应（PCR）的限制性内切酶消化方法对ACE和AGT基因多态性进行鉴定。两个试验组与对照组之间M235T和T174M变体的分布没有显著差异。根据疾病严重程度进行亚组分析时，患者中也未发现相关性。此外，风险并不局限于携带ACE基因D等位基因和AGT的T235T的个体。M235T和T174M变体不会增加印度人群患CHD或高血压的风险。
