Rana Garima, Yadav Suniti, Joshi Shipra, Saraswathy K N
Department of Anthropology, University of Delhi, New Delhi, 110007, India.
J Community Genet. 2018 Jan;9(1):51-55. doi: 10.1007/s12687-017-0321-9. Epub 2017 Aug 2.
Hypertension, a major risk factor for cardiovascular diseases, is among the leading causes of morbidity and mortality worldwide. Genetic predisposition to the risk of developing hypertension due to angiotensin-converting enzyme (ACE) gene insertion(I)/deletion(D) polymorphism (through altered serum ACE activity) is well documented among various populations. The present study investigated the possible association between ACE (DD) genotype and hypertension using a nested case-control study design including 451 individuals (of either sex in the age group 30-65 years) from a rural North Indian population practicing agriculture and lacto-vegetarianism. Blood Pressure was classified using JNC-7 criterion. Age- and sex-matched individuals were selected from normotensive (N-122), pre-hypertensive (N-123), hypertensive not on medication (N-122), and hypertensive on medication (N-84) categories. Amplification of DNA and genotyping of PCR product was done using standard protocols. From the analysis, comparatively higher frequency of individuals with DD genotype in the hypertensive category was observed, indicating a possible relation between DD genotype and hypertension. The odds ratio analysis revealed 2.225 (1.13-4.37)-fold significant increased risk for hypertension among cases, validating the vulnerability of individuals with DD genotype towards hypertension. Thus, the present study highlights the increased risk for developing hypertension due to ACE DD genotype in the studied population.
高血压是心血管疾病的主要危险因素之一，是全球发病和死亡的主要原因。血管紧张素转换酶（ACE）基因插入（I）/缺失（D）多态性（通过改变血清ACE活性）导致高血压风险的遗传易感性在不同人群中已有充分记录。本研究采用巢式病例对照研究设计，对来自印度北部农村从事农业和素食主义的451名个体（年龄在30 - 65岁之间，男女不限）进行了研究，以调查ACE（DD）基因型与高血压之间的可能关联。血压按照美国国家联合委员会第7次报告（JNC - 7）标准进行分类。从血压正常（N = 122）、高血压前期（N = 123）、未服药的高血压患者（N = 122）和正在服药的高血压患者（N = 84）类别中选取年龄和性别匹配的个体。DNA扩增和PCR产物基因分型按照标准方案进行。通过分析，观察到高血压类别中DD基因型个体的频率相对较高，表明DD基因型与高血压之间可能存在关联。优势比分析显示，病例组中患高血压的风险显著增加了2.225（1.13 - 4.37）倍，证实了DD基因型个体易患高血压。因此，本研究强调了在所研究人群中，ACE DD基因型会增加患高血压的风险。
