Neely A N, Clendening C E, Gardner J, Greenhalgh D G
Shriners Hospitals for Children, Shriners Burns Hospital, Cincinnati, Ohio 45229, USA.
J Burn Care Rehabil. 2000 Sep-Oct;21(5):395-402. doi: 10.1097/00004630-200021050-00001.
The gelatinases, matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9), have been implicated in different aspects of wound repair. However, little is known about MMP-2 and MMP-9 activity in animal models of impaired wound healing. We sought to compare serial gelatinase activities for 25 days after full-thickness excisional wounds in genetically diabetic healing-impaired mice and their nondiabetic non-healing-impaired littermates. Wound samples were frozen, homogenized, clarified by centrifugation, and analyzed on zymography gels, and MMP bands were quantitated relative to a conditioned media standard from HT-1080 cells. Gelatinase activity in both diabetic mice and nondiabetic mice increased after the mice were wounded. However, levels of latent gelatinases peaked earlier in the diabetic wounds, and there was more active MMP-2 and MMP-9 in the wounds of the diabetic mice than in the wounds of the nondiabetic mice. Because the higher gelatinase activity in the wounds of the diabetic mice was similar to the higher levels of gelatinase reported in difficult-to-heal wounds such as ulcers and burns, this diabetic mouse model may be useful for studies of these proteinases and their inhibitors in impaired wound healing.
明胶酶，即基质金属蛋白酶-2和-9（MMP-2和MMP-9），与伤口修复的不同方面有关。然而，在伤口愈合受损的动物模型中，关于MMP-2和MMP-9的活性了解甚少。我们试图比较基因性糖尿病愈合受损小鼠及其非糖尿病非愈合受损同窝小鼠全层切除伤口后25天内的系列明胶酶活性。将伤口样本冷冻、匀浆、离心澄清，然后在酶谱凝胶上进行分析，MMP条带相对于HT-1080细胞的条件培养基标准进行定量。受伤后，糖尿病小鼠和非糖尿病小鼠的明胶酶活性均增加。然而，潜伏性明胶酶水平在糖尿病伤口中更早达到峰值，并且糖尿病小鼠伤口中的活性MMP-2和MMP-9比非糖尿病小鼠伤口中的更多。由于糖尿病小鼠伤口中较高的明胶酶活性与溃疡和烧伤等难愈合伤口中报道的较高明胶酶水平相似，这种糖尿病小鼠模型可能有助于研究这些蛋白酶及其抑制剂在伤口愈合受损中的作用。
