Zini A, Nam R K, Mak V, Phang D, Jarvi K
Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada.
Fertil Steril. 2000 Oct;74(4):824-7. doi: 10.1016/s0015-0282(00)01495-3.
To examine and compare the effects of density-gradient centrifugation on the integrity of sperm DNA from the semen of both fertile and infertile men.
Prospective, observational study.
University infertility clinic.
Forty-four nonazoospermic, infertile men and nine fertile controls.
Semen samples were processed by density-gradient centrifugation. Sperm motility and sperm chromatin structure (evaluated by flow cytometry analysis of acridine orange-treated spermatozoa) were monitored before and after semen was processed.
Sperm motility and DNA integrity.
Following density-gradient centrifugation, mean sperm motility (+/-SEM) improved significantly compared to whole semen in samples from fertile and infertile men, respectively (71 +/- 6 vs. 49 +/- 7% and 56 +/- 3 vs. 44 +/- 3%, P<0.05). However, the percentage of sperm with denatured DNA increased compared to whole semen after processing of samples from infertile (25 +/- 3 vs. 15 +/- 2%, P<0. 01) but not fertile men (9 +/- 3 vs. 8 +/- 2%, P>0.05).
Our data indicate that the potential detrimental effect of density-gradient centrifugation on sperm DNA integrity is related to the initial semen quality. These data urge us to examine our current sperm-processing techniques to minimize sperm DNA damage.
检测并比较密度梯度离心对生育男性和不育男性精液中精子DNA完整性的影响。
前瞻性观察性研究。
大学不育症诊所。
44名非无精子症不育男性和9名生育对照者。
精液样本采用密度梯度离心法处理。在精液处理前后监测精子活力和精子染色质结构（通过吖啶橙处理精子的流式细胞术分析进行评估）。
精子活力和DNA完整性。
密度梯度离心后，生育男性和不育男性样本中精子的平均活力（±标准误）分别较全精液显著提高（71±6 vs. 49±7%以及56±3 vs. 44±3%，P<0.05）。然而，不育男性样本处理后DNA变性的精子百分比相比全精液增加（25±3 vs. 15±2%，P<0.01），而生育男性样本处理后DNA变性的精子百分比相比全精液未增加（9±3 vs. 8±2%，P>0.05）。
我们的数据表明，密度梯度离心对精子DNA完整性的潜在有害影响与初始精液质量有关。这些数据促使我们审视当前的精子处理技术，以尽量减少精子DNA损伤。
