Pirog E C, Kleter B, Olgac S, Bobkiewicz P, Lindeman J, Quint W G, Richart R M, Isacson C
Department of Pathology, Weill Medical College of Cornell University, New York, New York. Lenox Hill Hospital, New York, New York, USA.
Am J Pathol. 2000 Oct;157(4):1055-62. doi: 10.1016/S0002-9440(10)64619-6.
The prevalence of human papilloma virus (HPV) DNA in different histological subtypes of cervical adenocarcinoma and related tumors was examined using formalin-fixed, paraffin-embedded tissue samples from 105 primary cervical adenocarcinomas and adenosquamous carcinomas. Broad-spectrum HPV DNA amplification and genotyping was performed with the SPF10 primer set and line probe assay (LiPA), respectively. HPV DNA was detected in 82 of 90 (91%) mucinous adenocarcinomas, encompassing endocervical, intestinal, and endometrioid histological subtypes, and in nine of nine adenosquamous tumors (100%). HPV DNA was not detected in any nonmucinous adenocarcinomas including clear cell, serous, and mesonephric carcinomas (0/6). The most common viral types detected in adenocarcinoma were HPV 16 (50%) and HPV 18 (40%), followed by HPV 45 (10%), HPV52 (2%), and HPV 35 (1%). Multiple HPV types were detected in 9.7% of the cases. In conclusion, mucinous adenocarcinomas and adenosquamous carcinomas of the cervix demonstrate a very high prevalence of HPV DNA, similar to that reported for cervical squamous cell carcinoma. Only rare histological variants of cervical adenocarcinoma seem unrelated to HPV infection.
利用来自105例原发性宫颈腺癌和腺鳞癌的福尔马林固定、石蜡包埋组织样本,检测了人乳头瘤病毒(HPV)DNA在宫颈腺癌及相关肿瘤不同组织学亚型中的流行情况。分别使用SPF10引物组和线性探针分析(LiPA)进行广谱HPV DNA扩增和基因分型。在90例黏液腺癌(包括宫颈内膜、肠型和子宫内膜样组织学亚型)中的82例(91%)以及9例腺鳞癌中的9例(100%)检测到HPV DNA。在任何非黏液腺癌(包括透明细胞癌、浆液性癌和中肾管癌)中均未检测到HPV DNA(0/6)。腺癌中检测到的最常见病毒类型为HPV 16(50%)和HPV 18(40%),其次是HPV 45(10%)、HPV52(2%)和HPV 35(1%)。9.7%的病例检测到多种HPV类型。总之,宫颈黏液腺癌和腺鳞癌显示出非常高的HPV DNA流行率,与宫颈鳞状细胞癌的报道相似。宫颈腺癌中仅罕见的组织学变异型似乎与HPV感染无关。