Yamakawa Y, Forslund O, Teshima H, Hasumi K, Kitagawa T, Hansson B G
Department of Medical Microbiology, University of Lund, Malmö General Hospital, Sweden.
Gynecol Oncol. 1994 May;53(2):190-5. doi: 10.1006/gyno.1994.1114.
Although human papillomaviruses (HPV) have been implicated strongly in the pathogenesis of squamous cell carcinomas of the uterine cervix, a few studies have suggested that HPV may be an etiological factor for adenocarcinoma of the cervix. To evaluate the association between HPV infection and cervical adenocarcinoma, we analyzed formalin-fixed, paraffin-embedded tissues from 64 patients with adenocarcinoma or adenosquamous carcinoma of the uterine cervix for the presence of HPV DNA using polymerase chain reaction (PCR) with primers specific for the 6, 11, 16, 18, 31, 33, and 35 types. HPV DNA was detected in 24 of 43 (56%) cases of adenocarcinoma, and in 19 of 21 (91%) cases of adenosquamous carcinoma. Fifteen of the 43 (35%) adenocarcinomas and 10 of the 21 (48%) adenosquamous carcinomas contained HPV 18 DNA, and 10 of the 43 (23%) adenocarcinomas and 11 of the 21 (52%) adenosquamous carcinomas contained HPV 16 DNA. One specimen was positive for HPV 31 and five contained HPV 35 DNA, all but one as double infections with HPV 18. No evidence of HPV 6, 11, or 33 DNA carriage was seen. These results suggest that human papillomaviruses, particularly HPV 16 and 18, play a role in the etiology of cervical adenocarcinoma and adenosquamous carcinoma.
尽管人乳头瘤病毒（HPV）已被强烈认为与子宫颈鳞状细胞癌的发病机制有关，但一些研究表明HPV可能是子宫颈腺癌的一个病因。为了评估HPV感染与宫颈腺癌之间的关联，我们使用针对6、11、16、18、31、33和35型的引物，通过聚合酶链反应（PCR）分析了64例子宫颈腺癌或腺鳞癌患者的福尔马林固定、石蜡包埋组织中HPV DNA的存在情况。在43例腺癌病例中的24例（56%）以及21例腺鳞癌病例中的19例（91%）检测到了HPV DNA。43例腺癌中的15例（35%）和21例腺鳞癌中的10例（48%）含有HPV 18 DNA，43例腺癌中的10例（23%）和21例腺鳞癌中的11例（52%）含有HPV 16 DNA。一个标本HPV 31呈阳性，五个含有HPV 35 DNA，除一个外均为与HPV 18的双重感染。未发现HPV 6、11或33 DNA携带的证据。这些结果表明人乳头瘤病毒，尤其是HPV 16和18，在宫颈腺癌和腺鳞癌的病因学中起作用。
