Alian A, Sela-Donenfeld D, Panet A, Eldor A
Department of Virology, The Hebrew University-Hadassah Medical School, Jerusalem.
Virology. 2000 Oct 10;276(1):161-8. doi: 10.1006/viro.2000.0550.
Several years ago, a field strain retrovirus, avian hemangioma virus (AHV), was isolated from hemangioma tumors in layer hens. Sequence analysis indicated that the AHV genome contains the three prototypic retroviral genes, gag, pol, and env, and is devoid of an oncogene. In cultured endothelial cells, however, AHV induced a significant cytopathic effect through a typical apoptotic cascade. We now demonstrate that AHV also induces cell proliferation and anchorage-independent growth of BSC-1 epithelial cells and NIH-3T3 fibroblasts. This was shown by measurements of (1) cell viability, (2) DNA synthesis, (3) flow cytometry analysis of the cell DNA content, and (4) clonogenic efficiency of the infected cells. Anchorage-independent cell growth was demonstrated by colony formation in soft agar. Moreover, the AHV env gene was cloned into a MuLV-based retroviral vector, and infection of NIH-3T3 cells with this vector induced cell proliferation as well as clonogenic growth. These results suggest that AHV, which is devoid of an oncogene, is a pleiotropic activator capable of inducing either apoptosis or cellular proliferation, depending on the infected cell type.
几年前，一种野毒株逆转录病毒，即禽血管瘤病毒（AHV），从蛋鸡的血管瘤肿瘤中分离出来。序列分析表明，AHV基因组包含三个典型的逆转录病毒基因，即gag、pol和env，且不含癌基因。然而，在培养的内皮细胞中，AHV通过典型的凋亡级联反应诱导了显著的细胞病变效应。我们现在证明，AHV还能诱导BSC-1上皮细胞和NIH-3T3成纤维细胞的细胞增殖和不依赖贴壁的生长。这通过以下测量得以证明：（1）细胞活力、（2）DNA合成、（3）细胞DNA含量的流式细胞术分析以及（4）感染细胞的克隆形成效率。通过软琼脂中的集落形成证明了不依赖贴壁的细胞生长。此外，将AHV env基因克隆到基于鼠白血病病毒（MuLV）的逆转录病毒载体中，用该载体感染NIH-3T3细胞可诱导细胞增殖以及克隆生长。这些结果表明，不含癌基因的AHV是一种多效激活剂，能够根据感染的细胞类型诱导凋亡或细胞增殖。
