Feix G, Sano H
Eur J Biochem. 1975 Oct 1;58(1):59-64. doi: 10.1111/j.1432-1033.1975.tb02348.x.
The initiation specificity of RNA synthesis catalysed by the poly(C)-dependent Qbeta replicase activity was investigated with various synthetic ribopolymers as template. It was found that the initiation efficiency of a series of oligo(C) with various chain lengths is proportional to the template size. Synthetic riboheteropolymers containing cytidylic acid were accepted as templates only if they contained at their 3' end a cytidylic acid sequence of more than 5 nucleotides. Such an oligocytidylate sequence served also as an initiator sequence for copying the non-cytidylic-acid-containing part of the heterotemplate. RNA synthesis always began with the incorporation of GTP, even if the 3'-terminating nucleotide of the template was not cytidylic acid.
以各种合成核糖聚合物为模板，研究了由聚（C）依赖性Qβ复制酶活性催化的RNA合成起始特异性。发现一系列不同链长的寡聚（C）的起始效率与模板大小成正比。仅当含有胞苷酸的合成核糖杂聚物在其3'端含有超过5个核苷酸的胞苷酸序列时，才被接受为模板。这样的寡聚胞苷酸序列也作为复制杂模板中不含胞苷酸部分的起始序列。RNA合成总是从掺入GTP开始，即使模板的3'末端核苷酸不是胞苷酸。
