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不依赖结构的核苷酸序列分析

Structure-independent nucleotide sequence analysis.

作者信息

Mills D R, Kramer F R

出版信息

Proc Natl Acad Sci U S A. 1979 May;76(5):2232-5. doi: 10.1073/pnas.76.5.2232.

DOI:10.1073/pnas.76.5.2232
PMID:287063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC383572/
Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

摘要

在为RNA测序合成的核酸片段中，用肌苷替代鸟苷可有效防止电泳分析过程中二级结构的形成。因此，每个片段在测序凝胶中的迁移率严格取决于其分子量。肌苷替代应能显著提高DNA和RNA测序的分辨率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d7/383572/69170b8f979b/pnas00005-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d7/383572/754ed1b409e9/pnas00005-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d7/383572/69170b8f979b/pnas00005-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d7/383572/754ed1b409e9/pnas00005-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d7/383572/69170b8f979b/pnas00005-0152-a.jpg

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