Berzin V M, Gren E J
Biokhimiia. 1975 May-Jun;40(3):526-30.
The effect of synthetic polynucleotides and phage RNA on poly(C)-dependent synthesis of poly (G) by Qbeta replicase is studied. It is shown that single stranded poly(U) and poly (dT) are strong inhibitors whereas structured polynucleotides poly(A), MS2 RNA as well as double stranded complexes poly(A)-poly(U) and poly(A)-poly(dT) do not affect the synthesis of poly(G). It is suggested that contact region of template with enzyme has single stranded unhelical structure and affinity of polynucleotides to Qbeta replicase is determined by degree of their secondary structure.
研究了合成多核苷酸和噬菌体RNA对Qβ复制酶催化的依赖于聚（C）的聚（G）合成的影响。结果表明，单链聚（U）和聚（dT）是强抑制剂，而结构化多核苷酸聚（A）、MS2 RNA以及双链复合物聚（A）-聚（U）和聚（A）-聚（dT）不影响聚（G）的合成。提示模板与酶的接触区域具有单链非螺旋结构，多核苷酸与Qβ复制酶的亲和力由其二级结构程度决定。
