Xue Q, Renault T
Laboratoire de Génétique et Pathologie, IFREMER, La Tremblade, 17390, France.
J Invertebr Pathol. 2000 Oct;76(3):155-63. doi: 10.1006/jipa.2000.4965.
Enzymatic activities in the hemolymph of healthy and Bonamia-infected Ostrea edulis and Crassostrea gigas were studied with a commercial kit for the detection of 19 enzymes: 15 and 16 enzymes, respectively, were detected in the hemolymph of O. edulis and C. gigas and 10 of them showed relatively high activity levels. Most of them existed in both the cell-free fraction of the hemolymph and in the hemocytes. The cell-free hemolymph fraction of Bonamia ostreae-infected European flat oysters showed an elevated enzymatic activity level compared with that of healthy individuals. C. gigas hemocytes possessed higher enzymatic activity levels than O. edulis hemocytes. Differences in enzymatic activities existed in granulocytes and hyalinocytes in both oyster species. The enzyme release from oyster hemocytes seemed to be selective. The infection by B. ostreae induced enzymatic activity variations in European flat oysters. Higher enzyme levels within hemocytes may contribute partly to the natural resistance of C. gigas to the infection by B. ostreae.
利用一种用于检测19种酶的商用试剂盒，研究了健康的、感染博纳米虫的食用牡蛎和太平洋牡蛎血淋巴中的酶活性。在食用牡蛎和太平洋牡蛎的血淋巴中分别检测到15种和16种酶，其中10种酶表现出相对较高的活性水平。它们大多存在于血淋巴的无细胞部分和血细胞中。与健康个体相比，感染博纳米虫的欧洲扁牡蛎的无细胞血淋巴部分显示出较高的酶活性水平。太平洋牡蛎血细胞的酶活性水平高于食用牡蛎血细胞。两种牡蛎的粒细胞和透明细胞中存在酶活性差异。牡蛎血细胞中的酶释放似乎具有选择性。博纳米虫感染导致欧洲扁牡蛎的酶活性发生变化。血细胞中较高的酶水平可能部分有助于太平洋牡蛎对博纳米虫感染的天然抵抗力。
