da Silva P Mirella, Villalba Antonio
Centro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Aptdo. 13, E-36620 Vilanova de Arousa, Spain.
J Invertebr Pathol. 2004 Feb;85(2):97-104. doi: 10.1016/j.jip.2003.12.010.
A comparison among various histological techniques for the detection of the parasite Bonamia ostreae in oysters Ostrea edulis was performed to evaluate their sensitivity and suitability for different purposes. The comparison involved examination of histological sections, tissue imprints from gills, digestive gland, gonad and heart, and haemolymph cell monolayers, prepared through various protocols. Every technique produced some false negative. The haemolymph cell monolayers were more sensitive than tissue imprints and histological sections. Heart imprints provided the highest sensitivity among tissue imprints. Examination of histological sections was among the least sensitive techniques. Four procedures for estimation of infection intensity were compared. Some differences in accuracy for the estimation of infection intensity between haemolymph cell monolayers and histological sections (HS) were detected: there was a very good agreement when the infection appeared low or heavy in HS but it was not so good in the remaining cases. The results suggest the need for a critical review of the recommendations of the "Office Internationale des Epizooties" and the European Union for diagnosis of bonamiosis.
为评估不同组织学技术检测食用牡蛎（Ostrea edulis）中寄生虫奥氏博纳姆虫（Bonamia ostreae）的敏感性及对不同目的的适用性，对这些技术进行了比较。比较内容包括通过各种方案制备的组织切片、鳃、消化腺、性腺和心脏的组织印片以及血淋巴细胞单层的检查。每种技术都产生了一些假阴性结果。血淋巴细胞单层比组织印片和组织切片更敏感。心脏印片在组织印片中敏感性最高。组织切片检查是最不敏感的技术之一。比较了四种感染强度估计方法。检测到血淋巴细胞单层和组织切片（HS）在感染强度估计准确性上存在一些差异：当HS中的感染程度低或高时，两者一致性很好，但在其他情况下则不太好。结果表明，需要对“国际兽疫局”和欧盟关于博纳姆病诊断的建议进行严格审查。
