使用pH荧光蛋白进行突触前活动的光学测量。
The use of pHluorins for optical measurements of presynaptic activity.
作者信息
Sankaranarayanan S, De Angelis D, Rothman J E, Ryan T A
机构信息
Department of Biochemistry, The Weill Medical College of Cornell University, New York, New York 10021, USA.
出版信息
Biophys J. 2000 Oct;79(4):2199-208. doi: 10.1016/S0006-3495(00)76468-X.
Abstract
Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.
摘要
用于突触前活动光学测量的基因编码报告分子，在完整或半完整神经元网络内的神经传递测量方面具有巨大潜力。我们已经对基于pH敏感绿色荧光蛋白的神经末梢突触小泡循环传感器（pHluorins）进行了表征。pHluorins的pK约为7.1，这使其非常适合在动作电位期间通过质膜循环时追踪突触小泡腔的pH值。本文提供了使用这种方法对预期信号的理论分析以及未来报告分子开发的指导方针。
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本文引用的文献
1
Real-time measurements of vesicle-SNARE recycling in synapses of the central nervous system.中枢神经系统突触中囊泡-SNARE循环的实时测量。
Nat Cell Biol. 2000 Apr;2(4):197-204. doi: 10.1038/35008615.
2
In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons.深层皮质锥体神经元的体内树突钙动力学
Nat Neurosci. 1999 Nov;2(11):989-96. doi: 10.1038/14788.
3
Fluorescent proteins from nonbioluminescent Anthozoa species.来自非生物发光珊瑚虫纲物种的荧光蛋白。
Nat Biotechnol. 1999 Oct;17(10):969-73. doi: 10.1038/13657.
4
Membrane trafficking of neurotransmitter transporters in the regulation of synaptic transmission.
Trends Cell Biol. 1999 Sep;9(9):356-63. doi: 10.1016/s0962-8924(99)01605-0.
5
Inhibitors of myosin light chain kinase block synaptic vesicle pool mobilization during action potential firing.肌球蛋白轻链激酶抑制剂可在动作电位发放期间阻断突触小泡池的动员。
J Neurosci. 1999 Feb 15;19(4):1317-23. doi: 10.1523/JNEUROSCI.19-04-01317.1999.
6
Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy.通过荧光相关光谱法观察绿色荧光蛋白中荧光涨落的动力学
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13573-8. doi: 10.1073/pnas.95.23.13573.
7
The green fluorescent protein.绿色荧光蛋白。
Annu Rev Biochem. 1998;67:509-44. doi: 10.1146/annurev.biochem.67.1.509.
8
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Nature. 1998 Jul 9;394(6689):192-5. doi: 10.1038/28190.
9
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10
Regulation of dendritic growth and remodeling by Rho, Rac, and Cdc42.Rho、Rac和Cdc42对树突生长和重塑的调控
Neuron. 1997 Sep;19(3):625-34. doi: 10.1016/s0896-6273(00)80376-1.
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