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Synapto-pHluorins: chimeras between pH-sensitive mutants of green fluorescent protein and synaptic vesicle membrane proteins as reporters of neurotransmitter release.

作者信息

Yuste R, Miller R B, Holthoff K, Zhang S, Miesenböck G

机构信息

Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

出版信息

Methods Enzymol. 2000;327:522-46. doi: 10.1016/s0076-6879(00)27300-x.

DOI:10.1016/s0076-6879(00)27300-x
PMID:11045007
Abstract
摘要

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1
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Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo.突触结合蛋白I是体内代偿性突触小泡内吞作用所必需的。
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Synapto-pHluorins: genetically encoded reporters of synaptic transmission.突触pH荧光蛋白:突触传递的基因编码报告分子。
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Dual color detection of cyan and yellow derivatives of green fluorescent protein using conventional fluorescence microscopy and 35-mm photography.
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