肌球蛋白轻链激酶抑制剂可在动作电位发放期间阻断突触小泡池的动员。
Inhibitors of myosin light chain kinase block synaptic vesicle pool mobilization during action potential firing.
作者信息
Ryan T A
机构信息
Department of Biochemistry, The Weill Medical College of Cornell University, New York, New York 10021, USA.
出版信息
J Neurosci. 1999 Feb 15;19(4):1317-23. doi: 10.1523/JNEUROSCI.19-04-01317.1999.
Abstract
During repetitive action potential firing the maintenance of synaptic transmission relies on a continued supply of synaptic vesicles for fusion with the presynaptic plasma membrane. The mechanism of transport by which vesicles are delivered to the site of fusion from a reserve pool is unknown, as are the biochemical pathways linking intracellular Ca2+ elevation with vesicle mobilization. Here, using the fluorescent tracer FM1-43 in hippocampal synaptic terminals, I show that inhibitors of myosin light chain kinase can block mobilization of the reserve pool and not the immediately releasable pool.
摘要
在重复动作电位发放期间，突触传递的维持依赖于持续供应突触小泡以与突触前质膜融合。小泡从储备池被转运至融合位点的转运机制尚不清楚，将细胞内钙离子升高与小泡动员联系起来的生化途径也不清楚。在此，我利用荧光示踪剂FM1-43在海马突触终末进行研究，结果表明肌球蛋白轻链激酶抑制剂能够阻断储备池的动员，而不是立即可释放池的动员。
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