Ryan T A
Department of Biochemistry, The Weill Medical College of Cornell University, New York, New York 10021, USA.
J Neurosci. 1999 Feb 15;19(4):1317-23. doi: 10.1523/JNEUROSCI.19-04-01317.1999.
During repetitive action potential firing the maintenance of synaptic transmission relies on a continued supply of synaptic vesicles for fusion with the presynaptic plasma membrane. The mechanism of transport by which vesicles are delivered to the site of fusion from a reserve pool is unknown, as are the biochemical pathways linking intracellular Ca2+ elevation with vesicle mobilization. Here, using the fluorescent tracer FM1-43 in hippocampal synaptic terminals, I show that inhibitors of myosin light chain kinase can block mobilization of the reserve pool and not the immediately releasable pool.
在重复动作电位发放期间，突触传递的维持依赖于持续供应突触小泡以与突触前质膜融合。小泡从储备池被转运至融合位点的转运机制尚不清楚，将细胞内钙离子升高与小泡动员联系起来的生化途径也不清楚。在此，我利用荧光示踪剂FM1-43在海马突触终末进行研究，结果表明肌球蛋白轻链激酶抑制剂能够阻断储备池的动员，而不是立即可释放池的动员。
