果蝇转录延伸因子TFIIS编码基因的分离与特性分析。

Isolation and characterization of the gene encoding the Drosophila melanogaster transcriptional elongation factor, TFIIS.

作者信息

Oh Y, Yoon J, Baek K

机构信息

Department and Institute of Genetic Engineering, Kyunghee University, Suwon, South Korea.

出版信息

Biochim Biophys Acta. 1995 May 17;1262(1):99-103. doi: 10.1016/0167-4781(95)00055-l.

DOI:10.1016/0167-4781(95)00055-l

PMID:7772609

Abstract

We have characterized a genomic clone encoding the Drosophila melanogaster transcriptional elongation factor, TFIIS. The coding region of the TFIIS gene is interrupted by a short intron. The potential promoter region, deduced from the determination of the transcription start point (tsp), lacks distinct TATAAA or CCAAT box consensus sequences. Southern analysis and the in situ hybridization to chromosomes suggests that it is single-copy gene which is localized to the 35B region on the left arm of the second chromosome.

摘要

我们已对一个编码黑腹果蝇转录延伸因子TFIIS的基因组克隆进行了特征分析。TFIIS基因的编码区被一个短内含子打断。根据转录起始点（tsp）的测定推导出来的潜在启动子区域，缺乏明显的TATAAA或CCAAT框共有序列。Southern分析以及与染色体的原位杂交表明，它是一个单拷贝基因，定位于第二条染色体左臂的35B区域。