Willinger B, Apfalter P, Hirschl A M, Makristathis A, Rotter M, Seibold M
Dept. of Clinical Microbiology, Hygiene-Institute of University of Vienna, Austria.
Diagn Microbiol Infect Dis. 2000 Sep;38(1):11-5. doi: 10.1016/s0732-8893(00)00172-3.
Fungitest is a new commercially available and easy-to-perform breakpoint test system using six antifungal agents. We compared this test with a modified standard method described by the National Committee for Clinical Laboratory Standards (NCCLS). One hundred isolates of Candida species were tested with both methods. Based on the same breakpoints, the correlation of qualitative results between the reference method and Fungitest was high. Best results were obtained after incubation of Fungitest for 48 h. Overall agreement was high, an excellent correlation was given with amphotericin B and flucytosine (100% and 99%, respectively), whereas itraconazole showed only 86% concordance. When Fungitest was read after 24 h the agreement was lower ranging from 100% to 75%. Some of the breakpoints used with Fungitest differ from the breakpoints recommended by NCCLS, whereas others have not been elaborated by the NCCLS. The adaptation of Fungitest breakpoints to NCCLS and determination of further breakpoints have to be discussed before Fungitest can be recommended for routine use.
真菌测试（Fungitest）是一种新型的市售且易于操作的断点测试系统，它使用六种抗真菌剂。我们将该测试与美国国家临床实验室标准委员会（NCCLS）描述的改良标准方法进行了比较。用这两种方法对100株念珠菌属菌株进行了测试。基于相同的断点，参考方法与真菌测试之间的定性结果相关性很高。真菌测试在孵育48小时后获得了最佳结果。总体一致性很高，两性霉素B和氟胞嘧啶的相关性极佳（分别为100%和99%），而伊曲康唑的一致性仅为86%。当在24小时后读取真菌测试结果时，一致性较低，范围从100%到75%。真菌测试使用的一些断点与NCCLS推荐的断点不同，而其他一些断点NCCLS尚未详细说明。在推荐真菌测试用于常规使用之前，必须讨论将真菌测试断点调整为NCCLS的断点以及确定进一步的断点。
