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肺炎克雷伯菌泛酸缺陷型突变体将泛酸盐掺入柠檬酸裂解酶中。

Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae.

作者信息

Singh M, Dempsey W B, Srere P A

出版信息

J Bacteriol. 1975 Nov;124(2):686-92. doi: 10.1128/jb.124.2.686-692.1975.

DOI:10.1128/jb.124.2.686-692.1975
PMID:1102528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235955/
Abstract

A pantothenate-requiring mutant of Klebsiella pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase (3.4 mol/mol of enzyme). Analysis of a double-labeled enzyme ([14C]pantothenate and [3H]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3H and 14C were associated solely with the smallest subunit, the acyl carrier protein of citrate lyase.

摘要

分离出一株需要泛酸盐的肺炎克雷伯菌突变体。该突变体在生长时对泛酸盐表现出绝对依赖性。当在[14C]泛酸盐存在的情况下生长时，该突变体将[14C]泛酸盐掺入柠檬酸裂解酶中（每摩尔酶含3.4摩尔）。通过十二烷基硫酸钠凝胶电泳对双标记酶（[14C]泛酸盐和[3H]乙酸盐）进行分析表明，3H和14C都仅与最小的亚基即柠檬酸裂解酶的酰基载体蛋白相关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1db7/235955/2c06431794be/jbacter00324-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1db7/235955/2c06431794be/jbacter00324-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1db7/235955/2c06431794be/jbacter00324-0103-a.jpg

相似文献

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Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae.肺炎克雷伯菌泛酸缺陷型突变体将泛酸盐掺入柠檬酸裂解酶中。
J Bacteriol. 1975 Nov;124(2):686-92. doi: 10.1128/jb.124.2.686-692.1975.
2
The acyl-carrier protein of citrate lyase.柠檬酸裂解酶的酰基载体蛋白。
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Identification of a gene cluster in Klebsiella pneumoniae which includes citX, a gene required for biosynthesis of the citrate lyase prosthetic group.在肺炎克雷伯菌中鉴定出一个基因簇，其中包括citX，它是柠檬酸裂解酶辅基生物合成所需的基因。
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引用本文的文献

1
Structure of the prosthetic group of Klebsiella aerogenes citrate (pro-3S)-lyase.产气克雷伯菌柠檬酸（原-3S）裂解酶辅基的结构。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1872-6. doi: 10.1073/pnas.73.6.1872.

本文引用的文献

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Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.溶源性研究。I. 溶源性大肠杆菌释放噬菌体的方式。
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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Acetyl-CoA-dependent cleavage of citrate on inactivated citrate lyase.柠檬酸在失活的柠檬酸裂解酶上的乙酰辅酶A依赖性裂解。
Eur J Biochem. 1973 Aug 17;37(2):295-304. doi: 10.1111/j.1432-1033.1973.tb02988.x.
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Regulation of citrate lyase activity in Rhodopseudomonas gelatinosa.
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Translational control of specific uterine protein synthesis after estrogen induction.雌激素诱导后特定子宫蛋白合成的翻译调控。
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