DeAngelo A B, Fujimoto G I
Proc Natl Acad Sci U S A. 1973 Jan;70(1):18-21. doi: 10.1073/pnas.70.1.18.
The rate of leucine incorporation into a specific estrogen-induced protein of immature rat isolated by gel electrophoresis declines rapidly between 2-4 hr after estrogen stimulation in vivo followed by incubation in vitro. Actinomycin D present during the in vitro phase prevents this decline, and elicits a "superinduction" effect at 2 hr. Labeled induced protein remains stable during this time period, indicating that its decline is due to a reduction in synthesis capacity for the inducible protein, rather than to its degradation. A second injection of hormone at 3 hr has no effect on the reduced level of synthesis capacity for induced protein noted at 4 hr in the rat uterus.
通过凝胶电泳分离出的未成熟大鼠体内特定雌激素诱导蛋白中亮氨酸的掺入率，在体内雌激素刺激后2至4小时内迅速下降，随后进行体外孵育。体外阶段存在的放线菌素D可防止这种下降，并在2小时时引发“超诱导”效应。在此时间段内，标记的诱导蛋白保持稳定，这表明其下降是由于诱导蛋白合成能力的降低，而非其降解。在3小时时第二次注射激素，对4小时时大鼠子宫中诱导蛋白合成能力降低的水平没有影响。
