嗜热嗜酸栖热菌在酵母提取物中生长需求的多肽性质
Polypeptide nature of growth requirement in yeast extract for Thermoplasma acidophilum.
作者信息
Smith P F, Langworthy T A, Smith M R
出版信息
J Bacteriol. 1975 Nov;124(2):884-92. doi: 10.1128/jb.124.2.884-892.1975.
Abstract
The active component(s) in yeast extract required by Thermoplasma acidophilum for growth is polypeptide in nature. A fraction from yeast extract was isolated and partially characterized as one or more peptides of molecular weight about 1,000 containing 8 to 10 amino acids. Although it was composed largely of basic and dicarboxylic amino acids, only one amino group per molecule was free. The polypeptide(s) appeared to bind avidly to cations. No other organic compounds except glucose were needed by Thermoplasma. Among several hundred known compounds tested, only glutathione plus Fe2+ or Fe3+, clostridial ferredoxin, and spinach ferredoxin elicited any growth response.
摘要
嗜热栖热菌生长所需酵母提取物中的活性成分本质上是多肽。从酵母提取物中分离出了一个组分，并初步鉴定为一种或多种分子量约为1000、含8至10个氨基酸的肽。尽管它主要由碱性和二羧酸氨基酸组成，但每个分子只有一个氨基是游离的。该多肽似乎能与阳离子紧密结合。嗜热栖热菌除了葡萄糖外不需要其他有机化合物。在测试的数百种已知化合物中，只有谷胱甘肽加Fe2+或Fe3+、梭菌铁氧化还原蛋白和菠菜铁氧化还原蛋白能引起任何生长反应。
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